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happychemistChemistry news! Progress on two fronts this week!
One of my fronts is not yet ready to show, but I'm too proud to hold back on this one: Click for a low-resolution version of a pretty graph (H124).
What you see there is a spectrum of three things: a label, a protein, and a labeled protein. Which basically means that I've succeeded in attaching one to the other. Still need to confirm by mass spec that it's only one label per protein...but this is good news. And there aren't picogram quantities of this stuff. I think may even have enough to make measurements!
I'm now reading through papers in preparation for measurements, and determining which things I ought to measure and how to measure them...
One of my fronts is not yet ready to show, but I'm too proud to hold back on this one: Click for a low-resolution version of a pretty graph (H124).
What you see there is a spectrum of three things: a label, a protein, and a labeled protein. Which basically means that I've succeeded in attaching one to the other. Still need to confirm by mass spec that it's only one label per protein...but this is good news. And there aren't picogram quantities of this stuff. I think may even have enough to make measurements!
I'm now reading through papers in preparation for measurements, and determining which things I ought to measure and how to measure them...
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no subject
Date: 2005-10-21 03:48 pm (UTC)on a completely different chemistry topic
Date: 2005-10-23 07:42 pm (UTC)Treating aldehydes with Tollens' reagent (which is prepared by adding a drop of sodium hydroxide solution into silver nitrate solution to give a precipitate of silver(I) oxide, and then adding just enough dilute ammonia solution to redissolve the precipitate in aqueous ammonia to produce [Ag(NH3)2]+ complex) will convert aldehydes to carboxylic acids without attacking carbon-carbon double bonds. (See also oxidation of aldehyde.)
so i guess that has something to do with my ammonia + aldehyde question, but i don't really understand what it means.
Re: on a completely different chemistry topic
Date: 2005-10-23 07:47 pm (UTC)